In this section
@article{Nery:2026:femsle/fnag138,
author = {Nery, ET and Cardona, T and Avignone-Rossa, C and Sapienza, L and McFadden, J and Olaya-Castro, A and Jim茅nez, JI},
doi = {femsle/fnag138},
journal = {FEMS Microbiology Letters},
title = {Directed evolution and characterisation of light harvesting complexes with altered spectral properties in purple non-sulfur bacteria},
url = {http://dx.doi.org/10.1093/femsle/fnag138},
year = {2026}
}
TY - JOUR
AB - <jats:title>Abstract</jats:title> <jats:p>Purple non-sulfur bacteria (PNSB) are metabolically versatile microorganisms that inhabit diverse environments taking advantage of a remarkably efficient photosynthetic machinery. In this work, we describe the creation of a workflow for experimental studies aiming to compare the light absorption properties of structural variants of the light-harvesting complex 2 (LH2) of PNSB. Through the creation of a library of LH2 variants using site-directed mutagenesis, we engineered proteins with different spectral properties to be expressed in a LH2-defficient mutant of the model PNSB Rhodobacter sphaeroides. We validated this approach by reproducing a previously described mutant with a blue-shift in the B850 absorption peak, in addition to identifying a novel mutant exhibiting a red-shift of this peak. We characterised the fluorescence lifetime of the purified LH2 spectral variants in vitro. Our results suggest that directed evolution approaches can be used to systematically explore the landscape of light absorption in LH2s variants.</jats:p>
AU - Nery,ET
AU - Cardona,T
AU - Avignone-Rossa,C
AU - Sapienza,L
AU - McFadden,J
AU - Olaya-Castro,A
AU - Jim茅nez,JI
DO - femsle/fnag138
PY - 2026///
SN - 0378-1097
TI - Directed evolution and characterisation of light harvesting complexes with altered spectral properties in purple non-sulfur bacteria
T2 - FEMS Microbiology Letters
UR - http://dx.doi.org/10.1093/femsle/fnag138
UR - https://doi.org/10.1093/femsle/fnag138
ER -
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